当社のタンパク質高発現細胞作製技術の研究成果が「PLOS ONE」に掲載されました。
この度掲載されました論文は、当社と広島大学との共同研究で進めておりました「タンパク質高発現細胞IR/MAR（製品名：TG-Sure Expression）」に関する成果をまとめたものです。
Efficient Recombinant Production in Mammalian Cells Using a Novel IR/MAR Gene Amplification Method
Yoshio Araki, Tetsuro Hamafuji, Chiemi Noguchi and Noriaki Shimizu
Abstract
We previously found that plasmids bearing a mammalian replication initiation region (IR) and a nuclear matrix attachment region (MAR) efficiently initiate gene amplification and spontaneously increase their copy numbers in animal cells. In this study, this novel method was applied to the establishment of cells with high recombinant antibody production. The level of recombinant antibody expression was tightly correlated with the efficiency of plasmid amplification and the cytogenetic appearance of the amplified genes, and was strongly dependent on cell type. By using a widely used cell line for industrial protein production, CHO DG44, clones expressing very high levels of antibody were easily obtained. High-producer clones stably expressed the antibody over several months without eliciting changes in both the protein expression level and the cytogenetic appearance of the amplified genes. The integrity and reactivity of the protein produced by this method was fine. In serum-free suspension culture, the specific protein production rate in high-density cultures was 29.4 pg/cell/day. In conclusion, the IR/MAR gene amplification method is a novel and efficient platform for recombinant antibody production in mammalian cells, which rapidly and easily enables the establishment of stable high-producer cell clone.